منابع مشابه
Oxidative radioiodination damage to human lactoferrin.
Oxidative iodination of human lactoferrin (Lf) as commonly performed by using the chloramine-T, the Iodogen or the lactoperoxidase method produces an unreliable tracer protein because of excessive and heterogeneous polymer formation. Before iodination a minor tetramer fraction may be demonstrable in iron-saturated Lf only. Iodination-induced polymerization of iron-poor as well as iron-saturated...
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15 صفحه اولRadioiodination of sulfhydryl-sensitive proteins.
Radioiodination with rz51 has emerged as the method of choice for labeling protein to high specific radioactivity. A number of procedures have been developed to iodinate proteins under mild conditions and with reproducible results (l-7). Of these techniques, none is suitable for the iodination of proteins having thiol groups. Exposure to the oxidants Nchloro-p-toluene sulfonamide (chloramine-T)...
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We compared three different methods for radioiodination of human spleen ferritin. Tracers prepared by direct oxidative iodination with use of Chloramine T or 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril (Iodogen) were structurally altered by the labeling procedure, as was made apparent by gel filtration and electrophoresis on polyacrylamide gel. Tracers prepared by conjugation to rad...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1969
ISSN: 0021-9738
DOI: 10.1172/jci106009